| Applications | WB,ELISA |
|---|---|
| Species Cross-Reactivity | Human,Mouse,Rat |
| Molecular Weight | 56kDa |
| Source | Rabbit |
| Isotype | IgG |
| Recommended dilutions | WB :1:500-1:2000 ELISA: 1:5000-1:20000.Optimal dilutions should be determined by the end user |
| Immunogen | Synthesized peptide derived from human protein . at AA range: 430-510 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Specificity | DMP1 Polyclonal Antibody detects endogenous levels of protein. |
| Alternative Names | Dentin matrix acidic phosphoprotein 1 (DMP-1) (Dentin matrix protein 1) |
| Gene Id | |
| Swiss Prot No | |
| Subcellular Location | Nucleus . Cytoplasm . Secreted, extracellular space, extracellular matrix . In proliferating preosteoblasts it is nuclear, during early maturation stage is cytoplasmic and in mature osteoblast localizes in the mineralized matrix. Export from the nucleus of differentiating osteoblast is triggered by the release of calcium from intracellular stores followed by a massive influx of this pool of calcium into the nucleus. |
| Function | disease:Defects in DMP1 are the cause of autosomal recessive hypophosphatemic rickets (ARHR) [MIM:241520]. ARHR is characterized by rickets, osteomalacia, elevated FGF23 serum levels and hypophosphatemia.,function:May have a dual function during osteoblast differentiation. In the nucleus of undifferentiated osteoblasts, unphosphorylated form acts as a transcriptional component for activation of osteoblast-specific genes like osteocalcin. During the osteoblast to osteocyte transition phase it is phosphorylated and exported into the extracellular matrix, where it regulates nucleation of hydroxyapatite.,PTM:Phosphorylated in the cytosol and extracellular matrix and unphosphorylated in the nucleus. Phosphorylation is necessary for nucleocytoplasmic transport and may be catalyzed by a nuclear isoform of CK2 and can be augmented by calcium.,subcellular location:In proliferating preosteoblasts |
| Expression | Expressed in tooth particularly in odontoblast, ameloblast and cementoblast. |
| Background | Dentin matrix acidic phosphoprotein is an extracellular matrix protein and a member of the small integrin binding ligand N-linked glycoprotein family. This protein, which is critical for proper mineralization of bone and dentin, is present in diverse cells of bone and tooth tissues. The protein contains a large number of acidic domains, multiple phosphorylation sites, a functional arg-gly-asp cell attachment sequence, and a DNA binding domain. In undifferentiated osteoblasts it is primarily a nuclear protein that regulates the expression of osteoblast-specific genes. During osteoblast maturation the protein becomes phosphorylated and is exported to the extracellular matrix, where it orchestrates mineralized matrix formation. Mutations in the gene are known to cause autosomal recessive hypophosphatemia, a disease that manifests as rickets and osteomalacia. The gene structure is conser |
| Storage Buffer & Condition | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Store at -20°C. Stable for 12 months from date of receipt |
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