| Applications | WB, IHC |
|---|---|
| Species Cross-Reactivity | H,M,R |
| Molecular Weight | ~55KD |
| Source | Rabbit |
| Isotype | IgG |
| Recommended dilutions | WB: 1:5,000 IHC: 1:500-1,000 Optimal dilutions should be determined by the end user |
| Specificity | Theβtubulin Rabbit Polyclonal Antibody can detects endogenous β tubulin protein. |
| Alternative Names | Beta 4 tubulin, TBB5, TUBB2, TUBB2A, tubulin beta 2A, beta tubulin, tubulin beta chain |
| RRID | AB_3711205 |
| Background | Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 Da. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Tubulin may not be stable in certain cells. For example, expression of β-Tubulin in adipose tissue is very low and therefore β-Tubulin should not be used as loading control for these tissues. |
| Storage Buffer & Condition | PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50%Glycerol. Store at -20°C. Do not aliquot the antibody. |