| Applications | WB, IHC |
|---|---|
| Species Cross-Reactivity | Human,Mouse,Rat |
| Molecular Weight | 17kDa |
| Source | Mouse |
| Isotype | IgG1 |
| Recommended dilutions | WB: 1:500-1:1,000 IHC 1:100-200 Optimal dilutions should be determined by the end user |
| Specificity | Antibody can detects endogenous Cleaved Caspase 3 protein. |
| Alternative Names | APOPAIN antibody, CASP 3 antibody, CPP32 antibody, Cysterine protease CPP32 antibody |
| Background | Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires aspartic acid at the P1 position. |
| Storage Buffer & Condition | PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. Store at -20°C. Do not aliquot the antibody. |