| Applications | WB, IHC, IF |
|---|---|
| Species Cross-Reactivity | H, R, M |
| Molecular Weight | 29KD |
| Source | Mouse |
| Isotype | IgG1 |
| Recommended dilutions | WB: 1:5,000 IHC: 1:200 IF: 1:200 Optimal dilutions should be determined by the end user |
| Specificity | Antibody can detects endogenous PCNA protein. |
| RRID | AB_3711200 |
| Background | PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. It is found in the nucleus and is a cofactor of DNA polymerase delta. PCNA acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6 dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.
|
| Storage Buffer & Condition | PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50%Glycerol. Store at -20°C. Do not aliquot the antibody. |
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