| Applications | WB, IHC |
|---|---|
| Species Cross-Reactivity | H, R, M, Mk, Dg, C, Hm, Rb, Pg, Sh |
| Molecular Weight | 37KD |
| Isotype | IgG1 |
| Recommended dilutions | WB: 1:2,000-5,000 IHC: 1:100 Optimal dilutions should be determined by the end user |
| Specificity | The GAPDH antibody can detects endogenous GAPDH protein. |
| Background | Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively expressed in almost all tissues at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types. |
