| Applications | WB ,IHC |
|---|---|
| Species Cross-Reactivity | H, R, M, Mk, Dg, Ch, Hm, Rb, Pg, Sh |
| Molecular Weight | 43kDa |
| Isotype | IgG1 |
| Recommended dilutions | WB: 1:2,000-5,000 IHC: 1:100 Optimal dilutions should be determined by the end user |
| Specificity | Antibody can detects endogenous β-actin protein. |
| Background | β-actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, antibodies against β-actin are useful as loading controls for Western Blotting. However it should be noted that levels of β-actin may not be stable in certain cells. For example, expression of β-actin in adipose tissue is very low and therefore β-actin should not be used as loading control for these tissues. |
| Storage Buffer & Condition | PBS, pH 7.4, containing 0.02% kathon as Preservative and 50%Glycerol Store at -20°C. Do not aliquot the antibody. |
说明书.pdf
