| Applications | WB IP |
|---|---|
| Species Cross-Reactivity | N/A |
| Molecular Weight | N/A |
| Source | Rabbit |
| Isotype | IgG |
| Recommended dilutions | WB: 1:5,000 IP: 1:200 Optimal dilutions should be determined by the end user |
| Specificity | The Flag tag antibody can recognize C-terminal, internal, and N-terminal Flag-tag fusion proteins. |
| RRID | AB_3711191 |
| Background | The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence. |
| Storage Buffer & Condition | PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50%Glycerol. Store at -20°C. Do not aliquot the antibody. |
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