| Applications | WB, IP, IF,ELISA |
|---|---|
| Species Cross-Reactivity | N/A |
| Molecular Weight | N/A |
| Source | Mouse |
| Isotype | IgG1 |
| Recommended dilutions | WB: 1:5000 IP: 1:200 IF: 1:1000 ELISA:1:10000 Optimal dilutions should be determined by the end user |
| Specificity | The His tag antibody can recognize C-terminal, internal, and N-terminal His-tag fusion proteins. |
| RRID | AB_3711187 |
| Background | A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells. |
| Storage Buffer & Condition | PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50%Glycerol. Store at -20°C. Do not aliquot the antibody |
说明书.pdf
