| Applications | WB,IHC-p,IF,ELISA |
|---|---|
| Species Cross-Reactivity | Human,Mouse,Rat |
| Molecular Weight | 106kDa |
| Source | Rabbit |
| Isotype | IgG |
| Recommended dilutions | WB :1:500-1:2000 IHC-p: 1:100-1:300 IF: 1:200-1:1000 ELISA: 1:10000. Optimal dilutions should be determined by the end user |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Retinoblastoma around the phosphorylation site of Ser780. AA range:751-800 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Specificity | Phospho-Rb (S780) Polyclonal Antibody detects endogenous levels of Rb protein only when phosphorylated at S780. |
| Alternative Names | RB1; Retinoblastoma-associated protein; p105-Rb; pRb; Rb; pp110 |
| Gene Id | |
| Swiss Prot No | |
| Subcellular Location | Nucleus . During keratinocyte differentiation, acetylation by KAT2B/PCAF is required for nuclear localization. |
| Expression | Expressed in the retina. Expressed in foreskin keratinocytes (at protein level) |
| Storage Buffer & Condition | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Store at -20°C. Stable for 12 months from date of receipt |