| Applications | WB,IHC-p,IF,ELISA |
|---|---|
| Species Cross-Reactivity | Human,Mouse,Rat |
| Molecular Weight | 140kDa |
| Source | Rabbit |
| Isotype | IgG |
| Recommended dilutions | WB :1:500-1:2000 IHC-p: 1:50-1:300 IF: 1:50-1:300 ELISA: 1:10000. Optimal dilutions should be determined by the end user |
| Immunogen | The antiserum was produced against synthesized peptide derived from human KIF4A. AA range:1171-1220 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Specificity | KIF4A Polyclonal Antibody detects endogenous levels of KIF4A protein. |
| Alternative Names | KIF4A; KIF4; Chromosome-associated kinesin KIF4A; Chromokinesin-A |
| Gene Id | |
| Swiss Prot No | |
| Subcellular Location | Nucleus matrix . Cytoplasm . Cytoplasm, cytoskeleton, spindle . Midbody . Chromosome . Associates with chromosomes at all stage of mitosis (PubMed:11736643, PubMed:15297875, PubMed:15625105). Chromatin localization is dependent on iron-sulfur cluster binding (PubMed:29848660). In anaphase, associates with the mitotic spindle midzone (PubMed:15297875). In telophase and cytokinesis, co-localizes with CIAO2B at the spindle midzone and midbody (PubMed:29848660, PubMed:15297875). Co-localizes with PRC1 in early mitosis and at the spindle midzone from anaphase B to telophase (PubMed:15297875, PubMed:15625105). Does not localize to the nucleolus (PubMed:11736643). |
| Function | function:Motor protein that translocates PRC1 to the plus ends of interdigitating spindle microtubules during the metaphase to anaphase transition, an essential step for the formation of an organized central spindle midzone and midbody and for successful cytokinesis. May play a role in mitotic chromosomal positioning and bipolar spindle stabilization.,sequence caution:Contaminating sequence. Potential poly-A sequence.,similarity:Belongs to the kinesin-like protein family.,similarity:Belongs to the kinesin-like protein family. Chromokinesin subfamily.,similarity:Contains 1 kinesin-motor domain.,subcellular location:Not present in the nucleolus. In early mitosis, associated with the mitotic spindle, in anaphase, localized to the spindle midzone and, in telophase and cytokinesis, to the midbody. In late cytokinesis, found in the center of the midbody. Associated with chromosomes at all stag |
| Expression | Highly expressed in hematopoietic tissues, fetal liver, spleen, thymus and adult thymus and bone marrow. Lower levels are found in heart, testis, kidney, colon and lung. |
| Storage Buffer & Condition | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Store at -20°C. Stable for 12 months from date of receipt |
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