| Applications | WB,IHC-p,IF,ELISA |
|---|---|
| Species Cross-Reactivity | Human,Mouse,Rat |
| Molecular Weight | 26,35-65kDa |
| Source | Rabbit |
| Isotype | IgG |
| Recommended dilutions | WB :1:500-1:2000 IHC-p: 1:100-1:300 IF: 1:50-1:200 ELISA: 1:20000. Optimal dilutions should be determined by the end user |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human CD63. AA range:121-170 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Specificity | CD63 Polyclonal Antibody detects endogenous levels of CD63 protein. |
| Alternative Names | MLA1; TSPAN30; CD63 antigen; Granulophysin; Lysosomal-associated membrane protein 3; LAMP-3; Melanoma-associated antigen ME491; OMA81H;Ocular melanoma-associated antigen;Tetraspanin-30; Tspan-30; |
| Gene Id | |
| Swiss Prot No | |
| Subcellular Location | Cell membrane ; Multi-pass membrane protein . Lysosome membrane ; Multi-pass membrane protein . Late endosome membrane ; Multi-pass membrane protein . Endosome, multivesicular body . Melanosome . Secreted, extracellular exosome . Cell surface . Also found in Weibel-Palade bodies of endothelial cells (PubMed:10793155). Located in platelet dense granules (PubMed:7682577). Detected in a subset of pre-melanosomes. Detected on intralumenal vesicles (ILVs) within multivesicular bodies (PubMed:21962903). . |
| Function | function:This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation.,miscellaneous:Lack of expression of CD63 in platelets has been observed in a patient with Hermansky-Pudlak syndrome (HPS). Hermansky-Pudlak syndrome (HPS) is a genetically heterogeneous, rare, autosomal recessive disorder characterized by oculocutaneous albinism, bleeding due to platelet storage pool deficiency, and lysosomal storage defects. This syndrome results from defects of diverse cytoplasmic organelles including melanosomes, platelet dense granules and lysosomes. Ceroid storage in the lungs is associated with pulmonary fibrosis, a common cause of premature death in individuals with HPS.,similarity:Belongs to the tetraspanin (TM4SF) family.,subcellular location:Also found in Weibel-Palade bodies of endothelial cells. Located in platelet dense granules.,tissue |
| Expression | Detected in platelets (at protein level). Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages. |
| Storage Buffer & Condition | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Store at -20°C. Stable for 12 months from date of receipt |
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