Applications | WB,IHC |
---|---|
Species Cross-Reactivity | Human,Mouse,Rat |
Molecular Weight | 17kDa |
Source | Mouse |
Isotype | IgG1 |
Recommended dilutions | WB :1:500-1:1000 IHC: 1:100-1:200 Optimal dilutions should be determined by the end user |
Specificity | Antibody can detects endogenous Active Caspase 3 protein. |
Alternative Names | APOPAIN antibody, CASP 3 antibody, CPP32 antibody, Cysterine protease CPP32 antibody |
Background | Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires aspartic acid at the P1 position. |
Storage Buffer & Condition | PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. Store at -20°C. Stable for 12 months from date of receipt |